Scientist – Cellular Engineering and Assay Development

Description:

• Design and engineer Client cell lines in iPSCs, tumor cell lines, and other immortalized and primary cell types to support allogeneic cell therapy programs

• Generate stable engineered cell lines using lentiviral transduction and other viral vector systems

• Design genetic constructs and mRNA for stable and transient gene expression, including knock-in, knockout, and reporter strategies

• Perform transient mRNA-based expression to rapidly prototype and validate cell engineering strategies

• Establish clonal engineered cell lines through cell sorting (FACS) and single-cell cloning

• Develop, optimize, and execute cell-based assays to characterize engineered cells, including live-cell analysis on the Incucyte platform

• Design and run in-depth, multi-parameter flow cytometry panels to phenotype engineered cells and assess immune checkpoint receptor expression

• Evaluate the functional impact of genetic modification...